KMID : 0352720230470040524
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Journal of Ginseng Research 2023 Volume.47 No. 4 p.524 ~ p.533
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Ginsenosides Rg1 regulate lipid metabolism and temperature adaptation in Caenorhabditis elegans
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Hao Shi
Jiamin Zhao Yiwen L Junjie Li Yunjia Li Jia Zhang Zhantu Qiu Chaofeng Wu Mengchen Qin Chang Liu Zhiyun Zen Chao Zhang Lei Gao
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Abstract
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Background : Obesity is a risk factor for aging and many diseases, and the disorder of lipid metabolism makes it prominent. This study aims to investigate the effect of ginsenoside Rg1 on aging, lipid metabolism and stress resistance
Methods : Rg1 was administered to Caenorhabditis elegans (C. elegans) cultured in NGM or GNGM. The lifespan, locomotory activity, lipid accumulation, cold and heat stress resistance and related mRNA expression of the worms were examined. Gene knockout mutants were used to clarify the effect on lipid metabolism of Rg1. GFP-binding mutants were used to observe the changes in protein expression
Results : We reported that Rg1 reduced lipid accumulation and improved stress resistance in C. elegans. Rg1 significantly reduced the expression of fatty acid synthesis-related genes and lipid metabolism-related genes in C. elegans. However, Rg1 did not affect the fat storage in fat-5/fat-6 double mutant or nhr-49 mutant. Combined with network pharmacology, we clarified the possible pathways and targets of Rg1 in lipid metabolism. In addition, Rg1-treated C. elegans showed a higher expression of anti-oxidative genes and heat shock proteins, which might contribute to stress resistance
Conclusion : Rg1 reduced fat accumulation by regulating lipid metabolism via nhr-49 and enhanced stress resistance by its antioxidant effect in C. elegans.
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KEYWORD
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C. elegans, ginsenoside Rg1, lipid metabolism, stress resistance
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